In vitro Mutation breeding in Chrysanthemum

Abstract

Chrysanthemum (Chrysanthemum morifolium) is a globally significant ornamental plant valued for its diverse floral traits and adaptability. In vitro mutation breeding has emerged as a powerful tool for enhancing its genetic diversity and developing novel cultivars. This technique combines the advantages of tissue culture and induced mutagenesis to overcome the limitations of conventional breeding methods. Using physical mutagens (e.g., gamma rays) or chemical agents (e.g., ethyl methanesulfonate), targeted mutations can be introduced into explants such as shoot tips, petioles, or callus tissues under sterile conditions. Subsequent selection and regeneration in vitro facilitate the rapid screening of desirable traits, such as altered flower color, size, morphology, or improved abiotic and biotic stress tolerance. Advances in molecular markers and genomic tools have further streamlined the identification and characterization of mutations, enabling precision breeding. This approach not only accelerates the development of unique chrysanthemum varieties but also ensures sustainability by reducing dependency on natural resources. The integration of in vitro mutation breeding with advanced biotechnological methods holds immense potential for meeting market demands and addressing the challenges posed by climate change. Future research should focus on optimizing protocols, enhancing mutation efficiency, and exploring novel mutagenic agents for broader applications in chrysanthemum improvement.

DOI - https://doi.org/10.65525/SVUP.9788199956070.2026.92-102