ENVIRONMENTAL DNA (eDNA) METABARCODING’S POTENTIAL AND DIFFICULTIES FOR MANGROVE RESTORATION IN SOUTHEAST ASIA

Abstract

Mangrove forests in Southeast Asia are crucial for coastal protection, carbon sequestration, and biodiversity. However, rapid deforestation and habitat degradation necessitate efficient restoration strategies. Environmental DNA (eDNA) metabarcoding offers a non-invasive and cost-effective method for assessing biodiversity and monitoring ecosystem recovery. By analyzing genetic material from water and sediment samples, eDNA provides insights into species composition, including cryptic and rare organisms, which traditional surveys might overlook. This technology enables real-time tracking of restoration progress and ecosystem health. Despite its potential, eDNA metabarcoding faces challenges such as environmental degradation of DNA, taxonomic reference gaps, and data interpretation complexities. Factors like salinity, temperature, and microbial activity influence DNA persistence, potentially biasing results. Additionally, Southeast Asia’s diverse yet under-documented biodiversity limits the accuracy of genetic reference databases. Standardization of sampling protocols and bioinformatics pipelines is essential to ensure reliability across studies. Integrating eDNA with conventional monitoring can enhance mangrove restoration efforts by providing comprehensive biodiversity assessments. Addressing methodological challenges will be key to leveraging eDNA metabarcoding as a powerful tool for conservation and ecological restoration in Southeast Asia’s threatened mangrove ecosystems.

DOI - https://doi.org/10.65525/SVUP.9788198800473.2025.531-536