Blueprints of Life: Unlocking the Power of Modern Biotechnology
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ADVANCEMENTS IN PROBIOTIC ISOLATION AND CHARACTERIZATION: METHODS, MICROBIAL DIVERSITY, AND HEALTH BENEFIT

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  • Sriya Choudhury,
  • Satadru Ghosh,
  • Bidyut Bandyopadhyay,
  • Priyajit Banerjee
Sriya Choudhury
Laboratory of Drug Discovery and Protein Engineering, School of Life Sciences, Swami Vivekananda University, Barrackpore, Pin- 700121, West Bengal, India. Post Graduate Department of Biotechnology and Biochemistry, Oriental Institute of Science and Technology, Vidyasagar University, Midnapore, West Bengal, India.
Satadru Ghosh
Laboratory of Drug Discovery and Protein Engineering, School of Life Sciences, Swami Vivekananda University, Barrackpore, Pin- 700121, West Bengal, India.
Bidyut Bandyopadhyay
Post Graduate Department of Biotechnology and Biochemistry, Oriental Institute of Science and Technology, Vidyasagar University, Midnapore, West Bengal, India.
Priyajit Banerjee
Laboratory of Drug Discovery and Protein Engineering, School of Life Sciences, Swami Vivekananda University, Barrackpore, Pin- 700121, West Bengal, India.

Published 2025-12-03

Keywords

  • probiotics,
  • isolation,
  • characterization,
  • microbiome,
  • lactobacillus

How to Cite

Choudhury, Sriya, et al. “ADVANCEMENTS IN PROBIOTIC ISOLATION AND CHARACTERIZATION: METHODS, MICROBIAL DIVERSITY, AND HEALTH BENEFIT”. Swami Vivekananda University Press, Dec. 2025, pp. 209-24, https://swamivivekanandauniversitypress.com/index.php/svup/article/view/180.

Copyright (c) 2025 Swami Vivekananda University Press

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Abstract

Probiotics are live microbes that when administered in small amounts confer some health benefits to the host. Several different types of products which are formulated as drugs or other dietary supplements, originate from live microbes called probiotics. The majorities of these organisms belong to the LAB (Lactic Acid Bacteria) group. The LAB predominantly includes organisms which belong to the genera of Lactobacillus and Bifidobacterium but from varied species and strains which altogether is considered as potential probiotic. The aim of this review is to comprehensively explore the methodologies and advancements in the isolation and characterization of probiotics. This review investigates different methods for separating probiotics from a range of sources, including fermented foods, the human microbiome, and natural settings. It highlights the importance of cutting-edge methods like molecular biology, metagenomics, and whole genome sequencing in the identification of new probiotic strains, the description of their genetic and functional characteristics, and the evaluation of their possible health advantages.

DOI - https://doi.org/10.65525/SVUP.9788198800473.2025.209-224

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